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0KPYM_HUMAN*   SwissProt (?) | Description Local Annotation Link Reference
General Information
NamePKM2
DescriptionPyruvate kinase, isozymes m1/m2 (ec 2.7.1.40) (pyruvate kinase muscle isozyme) (cytosolic thyroid hormone-binding protein) (cthbp) (thbp1).
SpeciesHomo sapiens (NCBI taxonomy ID: 9606)
GO0005829 cytosol (NAS)
0005515 protein binding (IPI)
0004743 pyruvate kinase activity (TAS)
0006096 glycolysis (NAS)

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Domain Architecture (Details)
InterPro domains unassigned to SynO:
Pyruvate kinase () (PK) catalyses the final step in glycolysis .he conversion of phosphoenolpyruvate to pyruvate with concomitant phosphorylation of ADP to ATP: ADP + phosphoenolpyruvate = ATP + pyruvate The enzyme.hich is found in all living organisms.equires both magnesium and potassium ions for its activity . In vertebrates.here are four tissue-specific isozymes: L (liver). (red cells).1 (muscle.eart and brain).nd M2 (early foetal tissue). In plants.K exists as cytoplasmic and plastid isozymes.hile most bacteria and lower eukaryotes have one form.xcept in certain bacteria.uch as Escherichia coli.hat have two isozymes. All isozymes appear to be tetramers of identical subunits of ~500 residues.PK helps control the rate of glycolysis.long with phosphofructokinase () and hexokinase (). PK possesses allosteric sites for numerous effectors.et the isozymes respond differently.n keeping with their different tissue distributions . The activity of L-type (liver) PK is increased by fructose-1.-bisphosphate (F1.BP) and lowered by ATP and alanine (gluconeogenic precursor).herefore when glucose levels are high.lycolysis is promoted.nd when levels are low.luconeogenesis is promoted. L-type PK is also hormonally regulated.eing activated by insulin and inhibited by glucagon.hich covalently modifies the PK enzyme. M1-type (muscle.rain) PK is inhibited by ATP.ut F1.BP and alanine have no effect.hich correlates with the function of muscle and brain.s opposed to the liver.The structure of cat muscle pyruvate kinase has been determined . The protein comprises three domains each belonging to the alpha-beta class; one of these adopts a 3-layer(aba) sandwich architecture; the other two form beta-barrels.
  IPR001697:Pyruvate kinase
Pyruvate kinase () (PK) catalyses the conversion of phosphoenolpyruvate to pyruvate with the concomitant synthesis of ATP as the final step in glycolysis in both prokaryotes and eukaryotes . PK is a homotetramer.ach subunit consisting of four domains: a small N-terminal helical domain (absent in bacterial PK).n A domain with (beta/alpha)8-barrel topology. B domain with a beta-barrel topology.nd which is inserted within the A domain.nd a C-terminal with alpha/beta topology. This entry represents the B domain.The B domain of PK has structural similarity with the N-terminal domain of ATP sulphurylase (ATPS).hich consists of an incomplete 6-stranded beta-barrel.here the last PK strand is absent. ATPSs are ubiquitous enzymes that catalyse the primary step of intracellular sulphate activation .
  IPR011037:Pyruvate kinase, beta-barrel-like
IPR001697:PK 
Evalue:-272.823913574219 
Location:41-394IPR001697:PK_C 
Evalue:-74.958610534668 
Location:408-528
SequencesProtein: KPYM_HUMAN (530 aa)
mRNA: NM_002654
Local Annotation
Synapse Ontology
Calcium release from RyR (Ryanodine Receptor) in the SR (Sarcoplasmic Reticulum) is activated by the calcium induced-calcium-release
sdb:0325 RyR-CICR  (Evidence:keywords)
KO assignmentK00873
  Level 3 annotation:
    pyruvate kinase
  Level 2 annotation:
    Glycolysis / Gluconeogenesis
    Pyruvate metabolism
    Carbon fixation
    Purine metabolism
    Insulin signaling pathway
    Type II diabetes mellitus
Loci Structure (Details)Loci index, Chromosomal location, Length, Possible relational loci clusterExon1: 243 residues, 70278423-70279151Exon2: 62 residues, 70279868-70280050Exon3: 57 residues, 70281848-70282015Exon4: 53 residues, 70286122-70286275Exon5: 52 residues, 70286521-70286672Exon6: 92 residues, 70288015-70288286Exon7: 64 residues, 70289067-70289254Exon8: 46 residues, 70289741-70289873Exon9: 32 residues, 70296803-70296895Exon10: 57 residues, 70298338-70298505Exon11: 78 residues, 70310510-70310738Exon12: 2 residues, -Jump to KPYM_HUMAN  
Tune and view alternative isoforms
Loci Cluster (Details)Loci: 4178 70422832-70455393 ~-33K 12666(HEXA)(-)Loci: 4177 70278423-70310738 ~-32K 12655(PKM2)(-)Link out to UCSC