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0BPL1_HUMAN*   SwissProt (?) | Description Local Annotation Link Reference
General Information
NameHLCS
DescriptionBiotin--protein ligase (ec 6.3.4.-) (biotin apo-protein ligase) .
SpeciesHomo sapiens (NCBI taxonomy ID: 9606)
GO0004080 biotin-[propionyl-CoA-carboxylase (ATP-hydr... (TAS)
Domain Architecture (Details)
InterPro domains unassigned to SynO:
This domain is found in biotin protein ligase.ipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organism probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine . Lipoate-protein ligase A (LPLA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes .
  IPR004143:Biotin/lipoate A/B protein ligase
This C-terminal domain has an SH3-like barrel fold.he function of which is unknown. It is found associated with prokaryotic bifunctional transcriptional repressors and eukaryotic enzymes involved in biotin utilization . In Escherichia coli the biotin operon repressor (BirA) is a bifunctional protein. BirA acts both as the acetyl-coA carboxylase biotin holoenzyme synthetase (EC 6.3.4.15) and as the biotin operon repressor. DNA sequence analysis of mutations indicates that the helix-turn-helix DNA binding region is located at the N-terminus while mutations affecting enzyme function.lthough mapping over a large region.re found mainly in the central part of the proteins primary sequence .
  IPR003142:Biotin protein ligase, C-terminal
The biotin operon of Escherichia coli contains 5 structural genes involved in the synthesis of biotin. Transcription of the operon is regulated via one of these proteins.he biotin ligase BirA. BirA is an asymetric protein with 3 specific domains - an N-terminal DNA-binding domain. central catalytic domain and a C-terminal of unknown function. The ligase reaction intermediate.iotinyl-5-AMP.s the co-repressor that triggers DNA binding by BirA.The alpha-helical N-terminal domain of the BirA protein has the helix-turn-helix structure of DNA-binding proteins with a central DNA recognition helix. BirA undergoes several conformational changes related to repressor function and the N-terminal DNA-binding function is connected to the rest of the molecule through a hinge which will allow relocation of the domains during the reaction . Biotin-binding causes a large structural change thought to facilitate ATP-binding. Two repressor molecules form the operator-repressor complex.ith dimer formation occuring simultaneously with DNA binding. DNA-binding may also cause a conformational change which allows this co-operative interaction. In the dimer structure.he beta-sheets in the central domain of each monomer are arranged side-by-side to form a single.eamless beta-sheet. The apparent orthologs among the eukaryotes are larger proteins that contain a domain with high sequence homology to BirA.
  IPR004408:Biotin--acetyl-CoA-carboxylase ligase
IPR004143:BPL_LipA_LipB 
Evalue:-23.6575775146484 
Location:471-575IPR003142:BPL_C 
Evalue:-8.06048107147217 
Location:669-717
SequencesProtein: BPL1_HUMAN (726 aa)
mRNA: NM_000411
Local Annotation
Synapse Ontology
mitochondria are frequently observed in the vicinity of the synaptic vesicle clusters, in agreement with the ATP requirement of several steps of the vesicle cycle.
sdb:0118 mitochondria  (Evidence:keywords)
KO assignmentK01942
  Level 3 annotation:
    biotin-[methylmalonyl-CoA-carboxyltransferase] ligase
  Level 2 annotation:
    Biotin metabolism
K01943
  Level 3 annotation:
    biotin--[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase
  Level 2 annotation:
    Biotin metabolism
K01944
  Level 3 annotation:
    biotin-[methylcrotonoyl-CoA-carboxylase] ligase
  Level 2 annotation:
    Biotin metabolism
K01947
  Level 3 annotation:
    biotin-[acetyl-CoA-carboxylase] ligase
  Level 2 annotation:
    Biotin metabolism
K01949
  Level 3 annotation:
    
  Level 2 annotation:
    Other enzymes
Loci Structure (Details)Loci index, Chromosomal location, Length, Possible relational loci clusterExon1: 1176 residues, 37045062-37048588Exon2: 73 residues, 37050712-37050926Exon3: 40 residues, 37053897-37054012Exon4: 55 residues, 37059182-37059343Exon5: 24 residues, 37061388-37061456Exon6: 92 residues, 37191029-37191301Exon7: 63 residues, 37224420-37224603Exon8: 316 residues, 37230618-37231562Exon9: 56 residues, 37233001-37233164Exon10: 50 residues, 37240178-37240324Exon11: 47 residues, 37256205-37256340Exon12: 17 residues, 37284327-37284373Exon13: 2 residues, -Jump to BPL1_HUMAN  
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